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Assembly of the Mitochondrial Protein Import Channel Role of Tom5 in Two-Stage Interaction of Tom40 with the SAM Complex
Becker T, Guiard B, Thornton N, Zufall N, Stroud DA, Wiedemann N, Pfanner N.
Mol Biol Cell 21(18):3106-13.
We have identified a novel intermediate in the assembly pathway of Tom40, revealing a two-stage interaction of the precursor with the SAM complex. The second SAM stage represents assembly of Tom5 with the precursor of Tom40.
Mim1-deficient mitochondria accumulate Tom40 at the first SAM stage like Tom5-deficient mitochondria. Tom5 promotes formation of the second SAM stage and thus suppresses the Tom40 assembly defect of mim1 mitochondria.